Protein/DNA Interactions and Transfer Systems for the Receptor-Mediated Uptake of DNA

Nucleic acid transfer systems for the receptor-mediated uptake of DNA into the target cells are being developed on the basis of modularly-composed polypeptides and cationic lipoids. These gene-transfer systems contain specific modules for binding, protection and condensation of the nucleic acid, specific cell adhesion via receptors, aids for internalizing and for receptor mediated translocalization into the target cell. These totally synthetic systems allow new gene transfer strategies that are an alternative to the usual approach using retroviral and adenoviral recombinant vectors.

Publications:

1. K.Moelling, S.Volkmann, J.Dannull, A.Surovoy, and G. Jung (1993).
Analysis of Replication of HIV-1 by Mutagenesis of Reverse Transcriptase and Nucleocapsid Protein, Aids-Forschung (AIFO) 5, 243-248.

2. A. Yu. Surovoy, J. Dannull, K. Mölling, and G. Jung (1993).
Conformational and Nucleic Acid Binding Studies on the Synthetic Nucleocapsid Protein of HIV-1, J. Mol. Biol. 229, 94-104.

3. G. Müller, B. Strack, J. Dannull, B.S. Sproat, A. Surovoy, G. Jung, and K. Mölling (1994).
Amino Acid Requirements of the Nucleocapsid Protein of HIV-1 for Increasing Catalytic Activity of a Ki-ras Ribozyme in Vitro, J. Mol. Biol. 242, 422-429.

4. J. Dannull, A. Surovoy, G. Jung, and K. Mölling (1994).
Specific Binding of HIV-1 Nucleocapsid Protein to PSI-RNA in vitro Requires N-terminal Zinc Finger and Flanking Basic Amino Acid Residues, EMBO J. 13, 1525-1533.

cojinfo@uni-tuebingen.de(cojinfo@uni-tuebingen.de) - July 23, 1997